Icosagen

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Icosagen(總部:愛沙尼雅國)是一間專注於生物科技及生藥研究的委託研究機構,除了委托研究及客制生產服務外他們也會結合近年來的研究趨勢和客戶的需求開發最貼近市場需求的產品。Icosagen目前主要的產品包含以下幾個大類:抗體、蛋白質、ELISA 套組等等,此外他們也提供乳膠過敏反應及食品品質管理等相關的檢查服務。

Product News

Icosagen has released 5 new protein products for SARS-CoV-2 variant strains
P-314-100 SARS-CoV-2 Spike RBD2 South Africa VOC 501.V2 South Africa Variant
P-315-100 SARS-CoV-2 Spike RBD2 Brazil P1 Brazil Variant
P-316-100 SARS-CoV-2 Trimeric Spike South Africa VOC 501.V2 South Africa Variant Stable Trimer
P-317-100 SARS-CoV-2 Trimeric Spike UK VOC 202012/01 UK Variant Stable Trimer
P-318-100 SARS-CoV-2 Trimeric Spike Brazil P1 Brazil Variant Stable Trimer
Icosagen has released 3 new protein products for SARS-CoV-2 Research
P-309-100 SARS-CoV-2 Trimeric Spike Stable Trimer
P-310-100 SARS-CoV-2 Spike S1 UK VOC 202012/01 UK Variant
P-311-100 SARS-CoV-2 Spike RBD2 UK VOC 202012/01 UK Variant

核心技術

HybriFree 技術

Icosagen獨家的HybriFree 技術可以用於利用兔子或是雞來發現單株抗體。這個技術包括了獨立出生產抗體的細胞、選殖VH/VL序列以及建構供篩選用的人類IgG迷你資料庫。在抗體開發過程當中建立的表達載體可直接使用中國倉鼠卵巢細胞(CHO)進行重組抗體的生產製造。

HybriFree Technology

HybriFree技術的優點

  • 利用野生型兔子或是雞進行免疫反應能提供對應更多元樣的抗原表位的抗體且不需要親和力成熟 (Affinity maturation)也能有皮克摩爾等級的親和力
  • HybriFree的in vivo B細胞模式使其能獨立出擁有最理想生物化學特性之抗體,使之後的開發更加容易
  • 可以使用CHO細胞來表現以B細胞為基礎建構的IgG迷你資料庫,以達成高度的生產能力
  • 相較於老鼠,雞在演化分支上的距離更大,對於保守的序列及結構具有更強的免疫反應
  • HybriFree 抗體發現平台有Icosagen公司獨家QMCF發見技術及尖端的技術和深厚的生化經驗支撐,使他們得以生產開出高品質的抗原
  • Icosagen 獨家的VLP技術是他們能發現如GPCR等高純度的膜蛋白

兔子和雞作為免疫動物的優勢

老鼠
免疫反應時間較慢
親和性一般
業界標準

免疫反應時間最短
親和性優
最適合保守的目標
兔子
免疫反應時間較短
親和性最佳
最適合小分子目標

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QMCF技術

Icosagen的QMCF技術是基於在優化過的培養條件下在CHO細胞中複制並穩定表達載體來生產重組蛋白的技術。其通過對CHO細胞進行基因編輯使其表現下列兩種病毒因子來大幅縮短重組蛋白的生產時間及生產效率。

  • Polyomavirus Large T 抗原 (LT)
  • Epstein-Barr Virus EBNA1 蛋白

給客戶帶來的好處

  • 重組蛋白的生產(最大50 L規模)皆由博士學位級別的科學家進行管理,可以確保項目的順利進行(包括難以表現的蛋白質)、保持和客戶公開透明的聯絡、並可以提供詳細的項目報告
  • DNA表現載體的複製皆由不依靠連接酶的快速一步方式達成,其方法參考了Gibson assembly
  • 透過QMCF專利技術在CHO及HEK293細胞中表現蛋白可達成高成本效益及可擴展性
  • 可以在10天內生成用於診斷抗體生產用的QMCF CHO細胞庫和生產細胞庫
  • 可使用多種方法進行QC分析,包括蛋白質濃度,蛋白質印跡,PAGE,LabChip,IEF(以確定等電點),SEC分析(以確定聚集水平),LAL測試(以確定內毒素水平),Octet系統(以確定親和力和動力學),流動分析,DLS(以確定粒徑),HILIC HPLC(以確定岩藻糖基化水平),LC-MS和LC-MS / MS(確定蛋白質組學性質)等
  • 具有開發無標籤天然蛋白質的開發能力

Icosagen’s protein production service is based on the QMCF technology that is a robust mammalian expression system for recombinant proteins, delivering up to 1.5 g of antibody per litre of media. Expression vectors are constructed and transfected into CHO cells with our proprietary transfection reagent 007. Proteins are purified from the media with the method that is best suited for the protein of interest. Produced proteins and antibodies have high grade of purity, low endotoxin level and native folding. Deliverables include detailed reports.

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產品一覽

抗體



Nucleocapsid Protein SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-350-100 Nucleocapsid protein SARS-CoV-2 Chicken polyclonal IgY
R1-147-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-148-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-149-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-178-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-kappa
R1-179-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-kappa
Spike SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
R1-151-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-156-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-157-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-158-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-159-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-163-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-164-100 RBD protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-lambda
R1-169-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-170-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-180-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
BPV Type 1 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A1-100-100 BPV type 1 E2 Mouse monoclonal IgG
A1-101-100 BPV type 1 E2 Mouse monoclonal IgG
A1-150-100 BPV type 1 E2 Mouse monoclonal IgG
A1-151-100 BPV type 1 E2 Mouse monoclonal IgG
A1-152-100 BPV type 1 E2 Mouse monoclonal IgG
A1-153-100 BPV type 1 E2 Mouse monoclonal IgG
A1-154-100 BPV type 1 E2 Mouse monoclonal IgG
A1-155-100 BPV type 1 E2 Mouse monoclonal IgG
Ebolavirus GP Protein100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-100-100 Ebolavirus GP protein Rabbit polyclonal IgG
A2-101-100 Ebolavirus GP protein Monoclonal: rabbit scFv, human Fc hIgG1 scFv-Fc
HPV-18 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-401-100 HPV-18 E2 Mouse monoclonal IgG
HPV-11 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-402-100 HPV-11 E2 Mouse monoclonal IgG1
A2-403-100 HPV-11 E2 Mouse monoclonal IgG1
HPV-11 E7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-404-100 HPV-11 E7 Mouse monoclonal IgG2a
A2-405-100 HPV-11 E7 Mouse monoclonal IgG2a
HPV-18 E7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-406-100 HPV-18 E7 Mouse monoclonal IgG1
A2-407-100 HPV-18 E7 Mouse monoclonal IgG1
HCV 1b NS5B100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-420-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-421-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-422-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-423-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-424-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-425-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-426-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-427-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-428-100 HCV 1b NS5B Mouse monoclonal IgG1
C2-424-100 HCV 1b NS5B Partially humanized monoclonal IgG1
HIV-1 Nef100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-801-100 HIV-1 Nef Mouse monoclonal IgG1
A2-802-100 HIV-1 Nef Mouse monoclonal IgG1
A2-803-100 HIV-1 Nef Mouse monoclonal IgG1
A2-804-100 HIV-1 Nef Mouse monoclonal IgG1
A2-806-100 HIV-1 Nef Mouse monoclonal IgG1
A2-807-100 HIV-1 Nef Mouse monoclonal IgG1
A2-809-100 HIV-1 Nef Mouse monoclonal IgG1
A2-811-100 HIV-1 Nef Mouse monoclonal IgG1
HIV-1 Tat100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-821-100 HIV-1 Tat Mouse monoclonal IgG2b
A2-822-100 HIV-1 Tat Mouse monoclonal IgG1
A2-823-100 HIV-1 Tat Mouse monoclonal IgG1
A2-825-100 HIV-1 Tat Mouse monoclonal IgM
A2-826-100 HIV-1 Tat Mouse monoclonal IgG3
A2-827-100 HIV-1 Tat Mouse monoclonal IgG2b
HIV-1 Rev100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-832-100 HIV-1 Rev Mouse monoclonal IgG1
HIV-1 p24100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-851-100 HIV-1 p24 Mouse monoclonal IgG2a
A2-854-100 HIV-1 p24 Mouse monoclonal IgG1
A2-855-100 HIV-1 p24 Mouse monoclonal IgG1
A2-856-100 HIV-1 p24 Mouse monoclonal IgG2b
A2-857-100 HIV-1 p24 Mouse monoclonal IgG2a
A2-858-100 HIV-1 p24 Mouse monoclonal IgG1
A2-859-100 HIV-1 p24 Mouse monoclonal IgG1
C-851-100 HIV-1 p24 Partially humanized monoclonal IgG1
MLV p30100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A1-910-100 MLV p30 Rabbit polyclonal IgG
A2-301-100 MLV p30 Mouse monoclonal IgG
SIV-1 Nef100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-817-100 SIV-1 Nef Mouse monoclonal IgG1
Human GDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
315-100 Human  GDNF Mouse monoclonal murine IgG1
Human Artemin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
323-100 Human Artemin Mouse monoclonal IgG1
324-100 Human Artemin Mouse monoclonal IgG1
325-100 Human Artemin Mouse monoclonal IgG1
326-100 Human Artemin Mouse monoclonal IgG1
Human BDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
327-100 Human BDNF Mouse monoclonal IgG1
328-100 Human BDNF Mouse monoclonal IgG1
329-100 Human BDNF Mouse monoclonal IgG2b
Human CDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
300-100 Human CDNF Rabbit polyclonal IgG
301-100 Human CDNF Mouse monoclonal IgG1
302-100 Human CDNF Mouse monoclonal IgG1
Human MANF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
310-100 Human MANF Rabbit polyclonal IgG
311-100 Human MANF Mouse monoclonal IgG1
341-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
342-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
343-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
Human GFRa-1100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
303-100 Human GFRa-1 Chicken polyclonal IgY
Human GFRa-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
304-100 Human GFRa-2 Chicken polyclonal IgY
Human GFRa-3100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
305-100 Human GFRa-3 Chicken polyclonal IgY
Human GFRa-4100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
308-100 Human GFRa-4 Chicken polyclonal IgY
Human Neurturin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
307-100 Human Neurturin Rabbit polyclonal IgG
320-100 Human Neurturin Mouse monoclonal IgG1
321-100 Human Neurturin Mouse monoclonal IgG1
322-100 Human Neurturin Mouse Monoclonal IgG1
Human RET100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
306-100 Human RET Chicken polyclonal IgY
Hev b1100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-700-100 Hev b1 Mouse monoclonal IgG2a
A3-701-100 Hev b1 Mouse monoclonal IgG1
Hev b3100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-704-100 Hev b3 Mouse monoclonal IgG1
A3-705-100 Hev b3 Mouse monoclonal IgG1
Hev b5100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-707-100 Hev b5 Mouse monoclonal IgG1
A3-708-100 Hev b5 Mouse monoclonal IgG2b
Hev b6.02100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-709-100 Hev b6.02 Mouse monoclonal IgG1
A3-710-100 Hev b6.02 Mouse monoclonal IgG1
Rabbit Anti-Chicken Polyclonal Antibodies100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
903-100 chicken IgY Rabbit polyclonal, HRP conjugate IgG
904-100 chicken IgY Rabbit polyclonal, DyLight 488 conjugate IgG
905-100 chicken IgY Rabbit polyclonal, DyLight 549 conjugate IgG
Goat Anti-Mouse Polyclonal Antibodies100 μg
Catalogue # Immunogen Clonality Class
A1-900-100 Mouse IgG fraction Goat polyclonal HRP-conjugate IgG
A1-901-100 Mouse IgG fraction Goat polyclonal FITC-conjugate IgG
A1-902-100 Mouse IgG fraction Goat polyclonal, AP-conjugate IgG
Human C-C Motif Chemokine 20 (CCL20)100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
601-100 Human C-C motif chemokine 20 (CCL20) Mouse monoclonal IgG1
Human Elafin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
630-100 Human Elafin Mouse monoclonal IgG1
Human Interferon Lambda-4 (IFNL4)100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
600-100 Human Interferon lambda-4 (IFNL4) Rabbit polyclonal
Human S100-A8100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
620-100 Human S100-A8 Mouse monoclonal IgG1
Human S100-A9100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
610-100 Human S100-A9 Mouse monoclonal IgG1
Human Cardiac Troponin I100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
102-100 Human cardiac troponin I Mouse monoclonal IgG1
103-100 Human cardiac troponin I Mouse monoclonal IgG1
104-100 Human cardiac troponin I Mouse monoclonal IgG1
105-100 Human cardiac troponin I Mouse monoclonal IgG1
Recombinant Human Cardiac Troponin Complex100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
R1-126-100 Recombinant human cardiac troponin complex Recombinant human monoclonal Human IgG2-lambda
Anti-HEL Human IgG1 Isotype Control1000 μg
Catalogue # Immunogen Clonality Class
R1-146-000 Lysozyme from chicken egg white Recombinant human monoclonal IgG1
His-6 Tag100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-501-100 His-tag Mouse monoclonal IgG2b
Bovine DNase I100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
400-100 Bovine DNase I Rabbit polyclonal IgG
404-100 Bovine DNase I Mouse monoclonal IgG1
405-100 Bovine DNase I Mouse monoclonal IgG1
Human Ribonuclease 7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
406-100 Human Ribonuclease 7 Mouse monoclonal IgG1
407-100 Human Ribonuclease 7 Mouse monoclonal IgG1
408-100 Human Ribonuclease 7 Mouse monoclonal IgG1
Human Ribonuclease 8100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
430-100 Human Ribonuclease 8 Rabbit polyclonal IgG
Human Laminin a4100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-601-100 Human laminin a4 Mouse monoclonal IgG1
A2-604-100 Human laminin a4 Mouse monoclonal IgG1
A2-607-100 Human laminin a4 Mouse monoclonal IgG1
Human Laminin a5100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-610-100 Human laminin a5 Mouse monoclonal IgG1

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Proteins

SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Product Name Host
P-301-100 SARS-CoV-2 Nucleocapsid-His Protein CHO-based cell line (expressed by QMCF Technology)
P-302-100 Human recombinant ACE2-His Protein CHO-based cell line (expressed by QMCF Technology)
P-303-100 SARS-CoV-2 S1 RBD CHO-based cell line (expressed by QMCF Technology)
P-305-100 SARS-CoV-2 Spike S1 CHO-based cell line (expressed by QMCF Technology)
P-306-100 SARS-CoV-2 Spike S2 ECD-His CHO-based cell line (expressed by QMCF Technology)
P-307-100 SARS-CoV-2 S1 RBD2 CHO-based cell line (expressed by QMCF Technology)
P-308-100 Human recombinant ACE2-hFc CHO-based cell line (expressed by QMCF Technology)
P-309-100 SARS-CoV-2 Trimeric Spike CHO-based cell line (expressed by QMCF Technology)
P-310-100 SARS-CoV-2 Spike S1 UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-311-100 SARS-CoV-2 Spike RBD2 UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-314-100 SARS-CoV-2 Spike RBD2 South Africa VOC 501.V2 CHO-based cell line (expressed by QMCF Technology)
P-315-100 SARS-CoV-2 Spike RBD2 Brazil P1 CHO-based cell line (expressed by QMCF Technology)
P-316-100 SARS-CoV-2 Trimeric Spike South Africa VOC 501.V2 CHO-based cell line (expressed by QMCF Technology)
P-317-100 SARS-CoV-2 Trimeric Spike UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-318-100 SARS-CoV-2 Trimeric Spike Brazil P1 CHO-based cell line (expressed by QMCF Technology)
Others10 μg/50 μg/100 μg
Catalogue # Product Name Host
P-100-100 CDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-101-100 MANF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-102-100 Artemin, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-103-100 GDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-104-100 NGF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-105-100 BDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-120-100 GFRA-1-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-121-100 GFRA-2-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-122-100 GFRA-3-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-123-100 GFRA-4-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-124-100 RET-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-200-100 DNase I, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-201-100 DNase I, bovine recombinant CHO-based cell line (expressed by QMCF Technology)
P-300-100 Zaire Ebolavirus envelope glycoprotein Mammalian expression system
P-400-100 Recombinant human RNase 7 CHO-based cell line (expressed by QMCF Technology)
P-402-100 CD48, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-403-100 CD48, mouse recombinant CHO-based cell line (expressed by QMCF Technology)
P-404-100 2B4-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-405-100 2B4-His, mouse recombinant CHO-based cell line (expressed by QMCF Technology)
P-500-100 Recombinant murine leukemia virus p30 protein E. coli
P-600-100 Human Cardiac Troponin complex CHO-based cell line (expressed by QMCF Technology)

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ELISA Kits

SARS-CoV-296-well plate
Catalogue # Antibodies Against Detectable Antibodies Substrate
K5-001-096 SARS-CoV-2 IgG, IgM Antigen-coated (S1 and N) microplate wells
Natural Rubber Latex Allergen96-well plate
Catalogue # Product Name Limit of Detection Detection Range Manual MSDS
K3-350-020 FITkit® Hev b 1 ⩽ 2.0 μg/L 10-1000 μg/L Manual MSDS
K3-350-030 FITkit® Hev b 3 ⩽ 2.0 μg/L 10-1000 μg/L Manual MSDS
K3-350-040 FITkit® Hev b 5 ⩽ 1.5 μg/L 5-100 μg/L Manual MSDS
K3-350-010 FITkit® Hev b 6.02 (Hevein) ⩽ 1.5 μg/L 5-200 μg/L Manual MSDS
K3-350-050 ELISA Kit 4 Latex Allergens Hev b 1: 10-1000 µg/L
Hev b 3: 10-1000 µg/L
Hev b 5: 5-100 µg/L
Hev b 6.02: 5-200 µg/L
Manual
Others96-well plate
Catalogue # Product Name Limit of Detection Detection Range Manual MSDS
K2-001-096 Human CDNF ELISA Kit 1-8 pg/mL 15-960 pg/mL Manual
K2-002-096 Human Neurturin ELISA Kit 5-13 pg/mL 50-1600 pg/mL Manual
K2-003-096 Human Artemin ELISA Kit 2-8 pg/mL 30-1920 pg/mL Manual
K2-004-096 Human MANF ELISA Kit 30-75 pg/mL 0.25-16 ng/mL Manual
K4-001-096 Human RNase 7 ELISA kit 1-4 pg/mL 10-640 pg/mL Manual
K4-002-096 Human CD48 ELISA Kit 2-8 pg/mL 25-1600 pg/mL Manual
K4-003-096 Mouse CD48 ELISA Kit 6-16 pg/mL 50-3200 pg/mL Manual

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Transfection Reagents

Catalogue # Description
R007-P001 Reagent 007, 1 mg (powder)
R007-P010 Reagent 007, 10 mg (powder)
TC-010-000 Salmon Sperm Carrier DNA for transfection (10mg/mL) 1mL

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Services

Recombinant Antibody Production

Icosagen offers a broad range of tailored services for the development and production of recombinant antibodies, including complex antibody formats.

Our customers benefit from our cost effective QMCF Expression Technology that is based on the replication, and stable maintenance of the expression vectors in CHO cells, in conjunction with specifically optimized culture conditions for the recombinant antibody production.

There are two options for recombinant antibody production by using QMCF Technology:

  • Enhanced transient production of recombinant antibodies is used to deliver up to hundreds of milligrams of purified antibody within 3-4 weeks starting from vector cloning.
  • Stable episomal production is used to scale up the production volumes up to 40 liters cost effectively, and to deliver multiple gram quantities of purified antibody. In this case, also episomal production cell bank can be generated, which allows to omit transfection completely when repetitive and constant supply of the same antibody is required with consistent reproducibility.

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Antibody Development

Icosagen offers the following antibody development services:

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Protein Production

Icosagen provides recombinant protein production services using proprietary CHO-based QMCF Technology in serum-free conditions. Customer benefits are a short production time and cost-efficiency.

We offer two options for protein production:

  • Enhanced transient expression of recombinant proteins using high copy number transient pQMCF-T expression vectors. This option is fast, and a good fit for a small-scale pilot study to test the best expression conditions for your protein of interest.
  • Production using stable episomal production pools for large-scale production using pQMCF expression vectors that are stably maintained in proliferating CHO cells. This option is scalable and suitable for the cost effective production in up to 40 liter scale, and to produce protein of interest in up to gram-scale.

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Expression of Membrane Proteins

Production of VLP-s pseudotyped with membrane proteins

Icosagen produces VLPs pseudotyped with membrane proteins, such as ion-channels, receptors, viral glycoproteins, etc. For the formation of VLPs, we use retroviral (HIV-1 or MLV) and filoviral (ebola) proteins. VLPs could be produced in 293 or CHO-based QMCF cell lines.

  • Fast and efficient production of VLPs pseudotyped with different receptors, ion-channels, viral glycoproteins, etc.
  • Human-specific posttranslational modification pattern using 293-based cell line

Cell pool generation for membrane protein expression

Icosagen provides services for the fast development of CHO-based cell pools and cell lines, which express membrane proteins. The cell lines could be generated using QMCF Technology in 3 weeks, starting with the cloning of a protein of interest into a QMCF expression vector.

  • Fast and efficient way to generate a stable population of cells expressing a protein of interest; for investigation of signal transduction, screening of active compounds for early stages of drug development
  • Usage of CHO- or U2OS based adherent QMCF cell lines
  • Feasible generation of expression cell banks
  • Assay optimization and validation using Thermo Scientific ArrayScan VTI and appropriate Cellomics software

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若您對於頁面產品有任何疑問及需求,請與我們聯繫
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TEL : +886-2-25785958 FAX : +886-2-25789826

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